The purpose of this study was to characterize the antioxidant activity of 70% hydro-ethanolic extract prepared from the tuberous roots of Pueraria tuberosa Roxb.ex Willd. DC (PTE) using in vitro and in vivo experiments in rats. Material and methods: Presence of purerin, an isoflavone on PTE was quantified by HPTLC and standardized. The total phenolic content in PTE was calculated as Gallic acid equivalent (GAE) and reducing power as ascorbic acid equivalent (AAE). Anti-radical activity of PTE was examined as IC50 values using 1,1-diphenyl-2-picrylhydrazyl (DPPH), while, lipid peroxidation inhibition of PTE was assessed as IC50 values in rat brain homogenate. The effect of PTE (50, 100, 200 and 400 mg/kg, p.o. for 5 days) on brain lipid peroxidation and catalase in rats during hypoxia (30 min) were assessed spectrophotometrically. Results: HPTLC analysis revealed high content of isoflavone (purerin>6mg/g PTE). Remarkable phenolic content (GAE>5 µg/mg PTE), strong reducing ability (AAE>4mg/g PTE), anti-radical activity (IC50<32µg PTE) and lipid peroxidation (IC50<0.06µg PTE) was noted. Moreover, PTE significantly enhanced catalase (p<0.01) concentration and lowered the MDA formation (p<0.01) in brain during hypoxia induced stress in rats. Conclusion: The results indicated that tuberous root of Pueraria tuberosa for the utilization as significant source of natural antioxidant and to combat hypoxic stress.
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